Hippocampus anatomy rat
NSCs were acquired as previously described. Taken together, our results suggested that Runx1t1 was closely related to the neural differentiation in hippocampus. Moreover, upregulation of Runx1t1 promoted hippocampal neuronal differentiation both in vitro and in vivo. We found that Runx1t1 was strongly expressed in NSCs and weakly detectable in neurons in vitro and in vivo. In present work, the expression and distribution of Runx1t1 were detected during the differentiation of NSCs from rat hippocampus in vitro and in hippocampal DG in vivo. However, the exact function of Runx1t1 in neural development is largely unexplored. It was reported that Runx1t1 was involved in the proliferation and differentiation of hematopoietic stem cells. Runt-related transcription factor 1 translocated to 1 (Runx1t1) is one of the members of the MTG family. Some reports using biochemical and molecular analyses suggest that MTG family members act as downstream of proneural proteins to regulate stem cell proliferation and promote neuronal differentiation. MTG proteins have been reported to be sequentially expressed during neuronal differentiation and may promote the transition from precursor to neuron and induce the expression of neuronal genes in the differentiated cells. During the neurogenesis, a cascade of gene expression activated by proneural basic helix-loop-helix (bHLH) proteins plays an essential and conserved role in promoting neuronal differentiation, such as myeloid translocation genes (MTGs). Neurogenesis in the dentate gyrus (DG) of hippocampus can persist throughout the whole life, and it is also a multiple-step process and is regulated by several intrinsic and extrinsic factors. Thus, the identification of the factors and mechanisms underlying the neuronal differentiation of NSCs in guiding the production of NSCs for clinical needs is imperative. However, the usefulness of the strategies has been obstructed, especially, by limited neuronal differentiation of NSCs. Neurogenesis is a process by which newborn neurons are generated from neural stem cells (NSCs) or neural progenitor cells (NPCs) NSCs were considered to be a potential source of cells for cell replacement therapy during brain damage repair.